-Determining the three-dimensional structure of biomolecules in solutions, using internuclear geometric constraints (torsional angles, internuclear distances, RDCs).
-Determining the three-dimensional structure of ligands within complexes: ligand - protein, carbohydrate - protein, ligand - RNA/DNA, etc. (STD, Transfer NOE, Transfer RDC).
-Determining the structure of ligand receptors complexes.
-Determining the stability constants of biomolecular interactions.
-Analysis of mass variation close to a surface over which proteins are immobilized by modifying the composition of a solution.
-Determining association kA and dissociation kD kinetic parameters, enabling the calculation of the thermodynamic equilibrium constant (KD).
-The system allows the measuring of molecules surface affinities, and includes a CCD video camera to record images in real time from an array that can hold up to 400 Biomolecular interactions on the biochip functionalized surface.
Differential Scanning Calorimetry (DSC)
-Determining heats and temperatures of thermal transitions:
-Determining heat capacities and stability of dilute solutions.
-Analysis of biomolecular interactions.
Isothermal Titration Calorimetry (ITC)
-Determining dissociation constants and stoichiometry in binding phenomena:
-Determining the thermodynamic parameters for binding.
-Analysis of ionization phenomena.
-Determining micelle critical concentrations.
-Determining the molecular mass by detecting time of flight (TOF) with laser desorption ionization (MALDI), being possible to work in linear and reflectron mode.
-Determining the particle size ranging between 0.3 nm and 10 µm by Dynamic Light Scattering.
-Determining the molecular weights between 103 and 2 x 107 Dalton by Static Light Scattering.
-Determining the of zeta potential using M3-PALS technology.
Fast Protein Liquid Chromatography (FPLC)
-Purification platform for all forms of biomolecules using a combination of chromatography techniques.
•ENrich™ SEC 70 size exclusion column #7801070. Size range 0.5-70kDa, 10x300mm, 24 ml, 0.5–1.5 ml/min flow rate, maximum operating pressure 600psi.
•ENrich™ SEC 650 size exclusion column #7801650. Size range 5k–650kDa, 10x300mm, 24 ml, 0.75–2.0ml/min flow rate, maximum operating pressure 600psi.
•Foresight™ Nuvia™ S strong cation exchange column #7324740. 8x100mm, 5ml, 2,5-5ml/min flow rate, max operating pressure 435psi.
•Foresight™ Nuvia™ Q strong anion exchange column #7324741. 8x100mm, 5ml, 2,5-5ml/min flow rate, max operating pressure 435psi.
•Mini Profinity Affinity GST Cartridge #7324622. 40x12.6mm, 5ml, 5-10ml/min flow rate, maximum operating pressure 45 psi.
•Mini Nuvia™ Affinity IMAC Cartridges Ni charged column #7800811. 40x12.6mm, 5ml, 7ml/min flow rate, maximum operating pressure 45psi.
Inicio facturación del Servicio de FPLC
6 July 2018
Nuevo acceso a los servicios BIP
15 March 2018
Seminario avanzado de FPLC
9 March 2018
Facturación del Servicio de Maldi-Tof
2 May 2017
El equipo de RMN ya está activo
24 February 2017