NMR
-Determining the three-dimensional structure of biomolecules in solutions, using internuclear geometric constraints (torsional angles, internuclear distances, RDCs).
-Determining the three-dimensional structure of ligands within complexes: ligand - protein, carbohydrate - protein, ligand - RNA/DNA, etc. (STD, Transfer NOE, Transfer RDC).
-Determining the structure of ligand receptors complexes.
-Determining the stability constants of biomolecular interactions.
SPRi
-Analysis of mass variation close to a surface over which proteins are immobilized by modifying the composition of a solution.
-Determining association kA and dissociation kD kinetic parameters, enabling the calculation of the thermodynamic equilibrium constant (KD).
-The system allows the measuring of molecules surface affinities, and includes a CCD video camera to record images in real time from an array that can hold up to 400 Biomolecular interactions on the biochip functionalized surface.
Differential Scanning Calorimetry (DSC)
-Determining heats and temperatures of thermal transitions:
•Glass transitions
•"Cold" crystallization
•Phase changes
•Melting/unfolding
-Determining heat capacities and stability of dilute solutions.
-Analysis of biomolecular interactions.
Isothermal Titration Calorimetry (ITC)
-Determining dissociation constants and stoichiometry in binding phenomena:
•Protein-protein
•Protein-ligand
-Determining the thermodynamic parameters for binding.
-Analysis of ionization phenomena.
-Determining micelle critical concentrations.
-Reaction kinetics.
MALDI-TOF
-Determining the molecular mass by detecting time of flight (TOF) with laser desorption ionization (MALDI), being possible to work in linear and reflectron mode.
Light Scattering
-Determining the particle size ranging between 0.3 nm and 10 µm by Dynamic Light Scattering.
-Determining the molecular weights between 103 and 2 x 107 Dalton by Static Light Scattering.
-Determining the of zeta potential using M3-PALS technology.
Fast Protein Liquid Chromatography (FPLC)
-Purification platform for all forms of biomolecules using a combination of chromatography techniques.
-Service columns:
•ENrich™ SEC 70 size exclusion column #7801070. Size range 0.5-70kDa, 10x300mm, 24 ml, 0.5–1.5 ml/min flow rate, maximum operating pressure 600psi.
•ENrich™ SEC 650 size exclusion column #7801650. Size range 5k–650kDa, 10x300mm, 24 ml, 0.75–2.0ml/min flow rate, maximum operating pressure 600psi.
•Foresight™ Nuvia™ S strong cation exchange column #7324740. 8x100mm, 5ml, 2,5-5ml/min flow rate, max operating pressure 435psi.
•Foresight™ Nuvia™ Q strong anion exchange column #7324741. 8x100mm, 5ml, 2,5-5ml/min flow rate, max operating pressure 435psi.
•Mini Profinity Affinity GST Cartridge #7324622. 40x12.6mm, 5ml, 5-10ml/min flow rate, maximum operating pressure 45 psi.
•Mini Nuvia™ Affinity IMAC Cartridges Ni charged column #7800811. 40x12.6mm, 5ml, 7ml/min flow rate, maximum operating pressure 45psi.
Actualización precios de los Servicios BIP 2023
18 May 2023
Actualización precios de los Servicios BIP 2022
20 March 2022
Actualización precios Plataforma 2021
15 March 2021
New Scientific Lead of the Biomolecular Interactions Platform
21 May 2020
Actualización precios Plataforma 2020
13 April 2020
Actualización precios Plataforma y cambios en el servicio de RMN
11 January 2019
Nombramiento del Dr. Pedro Nieto como responsable científico de BIP
7 November 2018
Actualización tabla de precios de los servicios BIP
9 July 2018
Inicio facturación del Servicio de FPLC
6 July 2018
Nuevo acceso a los servicios BIP
15 March 2018
Seminario avanzado de FPLC
9 March 2018
Facturación del Servicio de Maldi-Tof
2 May 2017
El equipo de RMN ya está activo
24 February 2017