cicCartuja Centro de Investigaciones Científicas de la cartuja

  • Catalogue

Services Catalogue

 

NMR

— Determining the three-dimensional structure of biomolecules in solutions, using internuclear geometric constraints (torsional angles, internuclear distances, RDCs).  

— Determining the three-dimensional structure of ligands within complexes: ligand - protein, carbohydrate - protein, ligand - RNA/DNA, etc.  (STD, Transfer NOE, Transfer RDC).

— Determining the structure of ligand receptors complexes.

— Determining the stability constants of biomolecular interactions.

 

SPRi

— Analysis of mass variation close to a surface over which proteins are immobilized by modifying the composition of a solution.  

— Determining association kA and dissociation kD kinetic parameters, enabling the calculation of the thermodynamic equilibrium constant (KD).

— The system allows the measuring of molecules surface affinities, and includes a CCD video camera to record images in real time from an array that can hold up to 400 Biomolecular interactions on the biochip functionalized surface.

 

Differential Scanning Calorimetry (DSC) 

— Determining heats and temperatures of thermal transitions

• glass transitions

• "cold" crystallization

• phase changes

• melting/unfolding

— Determining heat capacities and stability of dilute solutions

— Analysis of biomolecular interactions

 

Isothermal Titration Calorimetry (ITC)

— Determining dissociation constants and stoichiometry in binding phenomena

• Protein-protein

• Protein-ligand

— Determining the thermodynamic parameters for binding

— Analysis of ionization phenomena

— Determining micelle critical concentrations

— Reaction kinetics

 

MALDI-TOF

— Determining the molecular mass by detecting time of flight (TOF) with laser desorption ionization (MALDI), being possible to work in linear and reflectron mode.  

 

Light Scattering

— Determining the particle size ranging between 0.3 nm and 10 µm by Dynamic Light Scattering.

— Determining the molecular weights between 103 and 2 x 107 Dalton by Static Light Scattering.  

— Determining the of zeta potential using M3-PALS technology.