cicCartuja Centro de Investigaciones Científicas de la cartuja

logo Junta de Andalucía logo Universidad de Sevilla logo CSIC

600 MHz NMR Spectrometer with cryoprobe

The spectrometer presents an UltraShieldPlus™ magnet featuring a high performance Cryoprobe. The 1H, 13C, 15N, and 19F Quadrupole Cryoprobe allows the recording of 1H-19F correlation with high sensitivity.


Surface Plasmon Resonance imaging

The system allows the measuring of molecules surface affinities, and includes a CCD video camera to record images in real time from an array that can hold up to 400 Biomolecular interactions.


Calorimetry

The equipment features two techniques: Differential Scanning Calorymetry (DSC) and Isotermal Titration Calorimetry (ITC). ITC is comprised of two equipments: one for low volume cells and a second for standard volume cells.


MALDI-TOF mass spectrometer

The bench-top MALDI-TOF equipped with reflectron enables the characterization of stable complexes with high precision. Thus the number of ligands with lower mass and 1:1 n:n complexes can be determined reliably.


Light Scattering

This technique measures particle sizes, molecular weights and zeta potentials to determine the aggregation state of samples, the hydrodynamic radius of macromolecules and particles, and the stoichiometry interaction in protein complexes.


Fast Protein Liquid Chromatography

The equipment is an Automated Medium-Pressure Chromatography System focused on biomolecule purification in cold room (4 ºC). The system controls and monitors: two Inlet Valve Modules, a Sample Pump Module, a Sample Inject Valve Module, a Pump Module, a Buffer Valve Module, an Air Sensor Module, two Columns Switching Valves Modules, a Multi- Wavelength Detector Module (UV-Vis and conductivity), a pH Valve Module, an Outlet Valve Module and a Fraction Collector.